Why Raman spectroscopy is a successful technique for bioprocess monitoring?

  • Optical vibrational spectroscopy technique characterizes molecular bonds and structure ⇒ very good selectivity
  • Aqueous medium doesn’t disturb Raman spectra (contrary to NIR spectroscopy)
  • In-situ measurement
  • The weakness of the Raman effect can be compensated by high power laser excitation of the moving medium, without risk of being destructive
  • Sensitivity still remains medium but fully sufficient for a measurement point every 10’ or much less with moving average technique
  • Fluorescence of culture medium is not a real issue in most of the cases